Actually the method requires only a single image to capture all the necessary data, multiple images can be used to reach a higher level of accuracy. We have been using this method succesfully for years now for several applications like photography, video, automotive, machine vision and others. Opt.The resolution measurement with sinusoidal Siemens stars is applicable to all digital cameras and done within minutes. Salido, ea., "MicroHikari3D: an automated DIY digital microscopy platform with deep learning capabilities," Biomed. AuthorĮrik Maddox (), Amsterdam Scientific Instruments B.V. Results representing the point spread function (PSF) and edge or line spread function (ESF or LSF) are also available.ĪSI_NNPS.ijm is a macro under development to calculate the NNPS based on a stack of flat field (open beam) images. The 2nd one is based on a Gaussian fit of the line spread data points for all pixels. The first one is based on a line spread histogram. The region of interest has to be sufficiently large to have enough data for the calculations. The edge does not have to be straight, however the macro needs one edge in the region of interest. A test image with several edges can be found in the data folder. The MTF as a function of pixel spatial frequency. Select a rectangular area around the edge and the macro will calculate Open an image file of a knife edge measurement. and open ASI_MTF.ijm to add this macro to the ImageJ plugin menu. Imagej macro (ASI_MTF.ijm) to calculate the modulation transfer function (MTF) based on a knife edge (or slanted edge) measurement.
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